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KMID : 0378019680110100065
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New Medical Journal
1968 Volume.11 No. 10 p.65 ~ p.69
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Effects of Caffeine and EDTA on the Red Cell Osmotic Fragility in Vitro
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姜福秀/Kang, Pock Soo
李尙鴻/李文杰/呂雄淵/Lee, Sang Hong/Lee, Moon Kul/Ryo, Ung Yun
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Abstract
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Caffeine, ethylene diamine tetra acetic. acid (EDTA) or sodium fluoride (NaF) was added in vitro to the blood sampled from normal dog. The concentration of each drug used was NaF; 0.025 M., caffeine; 0.017 M., and EDTA 0.025 M., and osmotic pressure was adjusted by addition of dextrose in appropriate amount.
Each drug solution in amount of 16.5 ml was added to the blood sample of 100 ml and osmotic fragility was measured in 0.45% 0.50%, and 0.55% saline solution respectively, at first, second and fourth hour` after the administration of drug to the blood.
For the measurement of osmotic fragility, one part of drug treated blood sample was mixed into 100 parts of.each saline solution and the amount of hemoglobin released from hemolyzed red cell was determined by means of photometric analysis.
Results obtained were analysed and compared with that of the control in which isotonic dextrose solution was added to the blood and all other experimental conditions given were same as those of drug treated blood.
Sodium fluoride increased red cell osmotic fragility significantly and the increase in fragility of EDTA. treated red. cells was more remarkable than that of NaF treated red cells, whereas caffeine showed no significant effect on red cell osmotic fragility.
In control and NaF treated blood, osmotic fragility tended to increase as the time progressed, on the other hand, osmotic fragility showed a tendency to decrease as the time "progressed in caffeine or EDTA treated blood, at 0.-55% saline solution particularly.
Loss of the lipopolysaccharide from cell wall layer23) and increase in permeability of cell membrane24) as an effect of EDTA on the membrane seemed to be responsible for the cause of increase in red cell fragility in _ EDTA treated blood. And as the caffeine has inhibitory effect on 3, 5´-phosphodiesterase26) inducing accumulation of 3´, 5´-AMP, authors were impressed _that caffeine may decrease red cell osmotic fragility enhancing the rate of glycolytic and some other metabolic processes.
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